How to use Dino-Lite’s DPQ feature to gather depth information
DPQ Depth Information Guide with compatible Edge models
DinoCapture 3.0 or 2.0 and selected Dino-Lite models offer an improved and more accurate functionality for the acquisition of depth information. Now, users can acquire depth information from the focus shift between two focal planes during live imaging.
This functionality only applies to the following compatible models that have EDOF:
| Series | Model |
|---|---|
| EdgePLUS | AM4917MZT, AM4917MZTL, AM4917MZT4, AM4917MT8, AM8917MZT, AM8917MZTL |
| Edge 5MP | AF7915MZT, AF7915MZTL |
| Edge 3.0 | AM73915MZT (R10), AM73915MZTL (R10A), AM73915MZT4, AM73915MT8 |
DinoCapture 3.0 or 2.0 ver. 1.5.40 or newer version required. Click here to download the latest version. Contact us for more information.
| Step | Reference |
|---|---|
| 1 Adjust the focus to a plane of interest. This position will be considered the reference plane as 0. |
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| 2 To acquire depth information, readjust the focus by: Middle-click (mousewheel click) on a point of interest to autofocus, if necessary, scroll the mouse wheel to re-adjust the focus. or Press the “Ctrl” key and scroll the mouse wheel to change focus manually. The depth information is shown as the focus shift. |
 or “Ctrl” +   This cursor  indicates that refocus mode is active and that the focus can be readjusted. A positive focus shift refers to a plane below the reference plane. |
| 3 To exit Refocus mode Left-click anywhere on the preview window or press the “ESC” key. |
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| Still have questions? We’re here to help. | Contact our technical support team |
Depth Acquisition: Maximum Depth Range and Accuracy
When performing depth acquisitions, its maximum depth range and tolerance are determined by the magnification being used. This section shows the variations in maximum depths and tolerance at different magnifications in AM4917 models.
Maximum Depth Range
The maximum measurable depths that the device can perform is referred to as maximum depth range. Usually, the higher the magnification, the lower the maximum depth range, and vice versa.
Tolerance
The tolerance is the allowable variance in depth acquisitions. The higher the magnification, the lower the tolerance, and vice versa.
Table with focus shift parameters of AM4917MZT and AM4917MZTL
Below is a table providing all the relevant parameters when acquiring depths with AM4917MZT and AM4917MZTL microscope models.
| AM4917MZT and AM73915MZT(R10) | |||
| Magnification | Max. Depth Range (+/- mm) (Down) | Max. Depth Range (+/- mm) (Up) | Tolerance (+/- mm) |
| 20 | 28.14 | 19.00 | 0.146 |
| 30 | 13.06 | 10.10 | 0.083 |
| 40 | 7.99 | 6.58 | 0.058 |
| 50 | 5.60 | 4.79 | 0.045 |
| 60 | 4.25 | 3.73 | 0.038 |
| 70 | 3.41 | 3.04 | 0.033 |
| 80 | 2.81 | 2.55 | 0.029 |
| 90 | 2.39 | 2.19 | 0.027 |
| 100 | 2.07 | 1.91 | 0.025 |
| 110 | 1.82 | 1.69 | 0.023 |
| 120 | 1.62 | 1.51 | 0.022 |
| 130 | 1.46 | 1.37 | 0.020 |
| 140 | 1.32 | 1.24 | 0.02 |
| 150 | 1.19 | 1.13 | 0.019 |
| 160 | 1.09 | 1.04 | 0.019 |
| 170 | 1.00 | 0.95 | 0.018 |
| 180 | 0.92 | 0.88 | 0.017 |
| 190 | 0.85 | 0.82 | 0.017 |
| 200 | 0.78 | 0.75 | 0.017 |
| 210 | 0.72 | 0.70 | 0.016 |
| 220 | 0.67 | 0.65 | 0.016 |
| AM4917MZTL and AM73915MZTL(R10A) | |||
| Magnification | Max. Depth Range (+/- mm) (Down) | Max. Depth Range (+/- mm) (Up) | Tolerance (+/- mm) |
| 20 | 34.94 | 25.25 | 0.251 |
| 30 | 16.89 | 13.62 | 0.149 |
| 40 | 10.65 | 9.04 | 0.108 |
| 50 | 7.59 | 6.65 | 0.087 |
| 60 | 5.83 | 5.22 | 0.074 |
| 70 | 4.69 | 4.26 | 0.065 |
| 80 | 3.91 | 3.59 | 0.059 |
| 90 | 3.34 | 3.09 | 0.054 |
| 100 | 2.90 | 2.70 | 0.051 |
| 110 | 2.55 | 2.40 | 0.048 |
| 120 | 2.28 | 2.16 | 0.046 |
| 130 | 2.05 | 1.94 | 0.044 |
| 140 | 1.84 | 1.75 | 0.042 |
